CUT&Tag2for1: a modified method for simultaneous profiling of the accessible and silenced regulome in single cells

CUT&Tag2for1: a modified method for simultaneous profiling of the accessible and silenced regulome in single cells

Blog Article

Abstract Cleavage Under Targets and Tagmentation (CUT&Tag) is an antibody-directed transposase tethering strategy for in situ grandpas best chromatin profiling in small samples and single cells.We describe a modified CUT&Tag protocol using a mixture of an antibody to the initiation form of RNA polymerase II (Pol2 Serine-5 phosphate) and an antibody to repressive Polycomb domains (H3K27me3) followed by computational signal deconvolution to produce high-resolution maps of both the active and repressive regulomes in single cells.The ability to seamlessly map active promoters, enhancers, and repressive regulatory elements using a single workflow provides a complete regulome profiling strategy suitable new belial model for high-throughput single-cell platforms.